LncRNA-NEAT1/miR-148a-3p axis regulates cell viability, apoptosis and autophagy through wnt/β-catenin signaling pathway in Breast Cancer


 Purpose: Breast cancer has over the years been one of major acute carcinomas in women. This study investigated fundamental mechanistic functions lncRNA-NEAT1/miR-148a-3p/Wnt/β-catenin axis moderating cell viability, apoptosis and autophagy Cancer (BC).
 Methods: RT-qPCR measured expression lncRNA NEAT1 microRNA-148a-3p human lines for Cancer. Cell transfection upregulated or silenced genes with CCK-8, western blot FCM assays determining cellular growth, proliferation protein related to biomarkers. Furthermore, luciferase assay analyzed activity lncRNA- microRNA-148a-3p
 Results: The outcomes indicated that LncRNA-NEAT1 was BC promoted inhibited Apoptosis cells. However, lncRNA-NEAT1 knockdown enhanced apoptosis. In addition, directly targeted microRNA-148a-3p. And, it found overturns inhibitory effects on imposed by overexpression. Lastly, overexpressed activated Wnt/β-catenin regulatory network through sponging lines.
 Conclusion: present showcased could enhance tumor development breast via playing role molecular sponge microRNA-148a-3p, eventually hyper invigorating network.